Inside Stanford's Genome Technology Center: Showcasing their high-throughput yeast genome editing setup
Wednesday 22 January 2025
PURPOSE
Where does genome editing technology stand today, and how can it evolve to meet modern research demands? Join us to learn how Kevin Roy’s lab has reached the milestone of selecting 1 million colonies, showcasing the immense power of integration in lab automation.
WHAT YOU'LL LEARN
How a leading genome editing technologist mastered the challenges of integrated lab automation.
The key considerations when implementing high-throughput CRISPR screens.
Proven strategies for achieving library construction at ultra high throughput.
SCHEDULE
05:00 - 05:40 PM (GMT) / 09:00 - 09:40 AM (PST) / 12:00 - 12:40 PM (EST) | Stanford Genome Technology Center's high-throughput colony picking setup - Kevin Roy
05:40 - 6:00 PM (GMT) / 09:40 - 10:00 AM (PST) / 12:40 - 01:00 PM (EST) | Q&A session
Kevin Roy, PhD
Dr. Kevin Roy is an established Research Scientist at Stanford University's Genome Technology Center. A key milestone in Kevin’s work has been to pioneer a "supercharged donor repair system" for saturation genome editing based on CRISPR technology.
By leveraging advancements in multiple library-scale guide-donor systems originally developed in yeast, the system designed by Kevin and his team allows for tens of thousands of yeast cells to undergo unique genetic edits simultaneously. Using proprietary genome-integrated barcode technology, these edits can be accurately tracked, providing a way to both identify and validate individual hits from a screen in a single experiment. The use of robotics for clone selection and library construction has substantially reduced experimental timelines and expedited time to publication.
Kevin’s insights therefore offer invaluable guidance for those interested in scaling their workflow using integrated lab automation solutions.
Harry Singer, PhD
Harry Singer is the CEO of Singer Instruments, a global leader in microbial benchtop lab automation solutions. He spearheaded the company’s strategic shift toward advanced robotics by partnering with the yeast genetics community and most recently synthetic biology innovators at SynCTI. Fostering direct collaboration between scientists and engineers led to development of ROTOR+, a revolutionary high throughput screening workstation and PIXL, a precision colony picker built for the specific needs of microbiologists.
Harry continues to champion community-driven design, responding to customer demand for fully walkaway colony picking with PIXL Dark, a robot arm-friendly version of PIXL that integrates with larger lab automation systems, supporting reliable walkaway workflows for streamlined research and productivity.